The nandb package gives functions for calculation of molecular number and
brightness from images, as detailed in Digman et al. 2008. It comes with an
implementation of the novel 'automatic detrending' technique.
Digman MA, Dalal R, Horwitz AF, Gratton E. Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope. Biophysical Journal. 2008;94(6):2320-2332. doi: 10.1529/biophysj.107.114645 .